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rabbit polyclonal anti erk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit polyclonal anti erk
    Rabbit Polyclonal Anti Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+phospho+erk/Phospho-p38+MAPK+(Thr180%2FTyr182)+Mouse+mAb/pmc12953758-313-74-77
    Average 96 stars, based on 1900 article reviews
    rabbit polyclonal anti erk - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: P2Y 2 deficiency impacts adult neurogenesis and related forebrain functions.
    Article Snippet: .. Membranes were incubated with rabbit polyclonal anti- phospho- AKT (1:2500, CST), rabbit polyclonal anti- phospho- ERK (1:2500, CST), mouse anti- b- Actin (1:5000, Santa Cruz, Paso Robles), or Rabbit polyclonal anti- ß- Tubulin (1:10 000, Abcam) over night at 4°C. .. After washing, membranes were incubated with secondary HRP- conjugated goat anti- rabbit IgG (1:40 000, Dianova) and HRP- conjugated goat anti- mouse IgG (1:5000, Carl Roth) for 1h at the room temperature.

    Article Title: CQMUH-011 Inhibits LPS-Induced Microglia Activation and Ameliorates Brain Ischemic Injury in Mice
    Article Snippet: An 8-μL sample of the protein was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, USA). .. The membranes were blocked for 1 h with 5% BSA at room temperature and then incubated with specific primary antibodies: rabbit polyclonal anti-ERK(1/2) (#9102, Cell Signaling Technology, MA, USA, 1:1000), rabbit polyclonal anti-phospho-ERK (1/2) (#4376, Cell Signaling Technology, MA, USA, 1:1000), mouse monoclonal anti -Phospho-NF-κB p65 (#13346, Cell Signaling Technology, MA, USA, 1:1000), mouse monoclonal anti-IκBα (#9247, Cell Signaling Technology, MA, USA, 1:1000), mouse monoclonal anti-phospho-IκBα (#9246, Cell Signaling Technology, MA, USA, 1:1000), rabbit polyclonal anti-p38 (#9212, Cell Signaling Technology, MA, USA, 1:1000), rabbit polyclonal anti-Phospho-p38 (#9211, Cell Signaling Technology, MA, USA, 1:1000), mouse monoclonal anti-PCNA(sc-53407, Santa Cruz, CA, USA, 1:1000), rabbit polyclonal anti-cyclin D1 (sc-717, Santa Cruz, CA, USA, 1:1000), mouse monoclonal anti-Bax (sc-70407, Santa Cruz, CA, USA, 1:1000), mouse monoclonal anti-Bcl-2 (sc-23960, Santa Cruz, CA, USA, 1:1000), rabbit polyclonal anti-TLR4 (sc-10741, Santa Cruz, CA, USA, 1:1000), rabbit polyclonal anti-MyD88 (sc-11356, Santa Cruz, CA, USA, 1:1000), and rabbit polyclonal anti-β-actin (20536-1-AP, Proteintech, Wuhan, China, 1:3000). ..

    Article Title: High glucose–induced Smad3 linker phosphorylation and CCN2 expression are inhibited by dapagliflozin in a diabetic tubule epithelial cell model
    Article Snippet: Equal amounts of protein (20/25 μg per sample) were boiled in 2× Laemmli buffer (Bio-Rad) with 5% mercaptoethanol for 5 min at 95°C and separated on either 4-15%, 7.5% or 10% ‘Mini-PROTEAN TGX Stain-Free’ gels (Bio-Rad) for ∼25 min at 220 V. Images of gel proteins were captured on the ChemiDocTM Touch system (Bio-Rad) before the transfer onto polyvinylidene (PVDF) membranes for total protein normalisation and adjustment for loading. .. Blots were blocked with 5% non-fat milk, in tris-buffered saline-tween (TBST), and incubated overnight at 4°C with rabbit polyclonal anti SGLT2 (1:500, Santa Cruz), rabbit polyclonal anti CCN2 (1:500, Invitrogen), rabbit polyclonal anti-phospho-ERK (1:1000, CST), rabbit polyclonal anti phospho-Smad3(SS p XS p ) (1:1000, CST), or rabbit polyclonal anti phospho-Smad3 LR serine 204 (1:1000, Abcam). .. Blots were washed in TBST three times and incubated with an anti-rabbit IgG horseradish peroxidase (HRP) conjugate secondary antibody for an hour at room temperature (1:2000, New England Biolabs).

    other:

    Article Title: GFAT2 mediates cardiac hypertrophy through HBP-O-GlcNAcylation-Akt pathway
    Article Snippet: Rabbit polyclonal anti-phospho-ERK , Cell Signaling Technology , Cat#9101.

    Saline:

    Article Title: High glucose–induced Smad3 linker phosphorylation and CCN2 expression are inhibited by dapagliflozin in a diabetic tubule epithelial cell model
    Article Snippet: Equal amounts of protein (20/25 μg per sample) were boiled in 2× Laemmli buffer (Bio-Rad) with 5% mercaptoethanol for 5 min at 95°C and separated on either 4-15%, 7.5% or 10% ‘Mini-PROTEAN TGX Stain-Free’ gels (Bio-Rad) for ∼25 min at 220 V. Images of gel proteins were captured on the ChemiDocTM Touch system (Bio-Rad) before the transfer onto polyvinylidene (PVDF) membranes for total protein normalisation and adjustment for loading. .. Blots were blocked with 5% non-fat milk, in tris-buffered saline-tween (TBST), and incubated overnight at 4°C with rabbit polyclonal anti SGLT2 (1:500, Santa Cruz), rabbit polyclonal anti CCN2 (1:500, Invitrogen), rabbit polyclonal anti-phospho-ERK (1:1000, CST), rabbit polyclonal anti phospho-Smad3(SS p XS p ) (1:1000, CST), or rabbit polyclonal anti phospho-Smad3 LR serine 204 (1:1000, Abcam). .. Blots were washed in TBST three times and incubated with an anti-rabbit IgG horseradish peroxidase (HRP) conjugate secondary antibody for an hour at room temperature (1:2000, New England Biolabs).



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